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KMID : 0352719930170010039
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Journal of Ginseng Research
1993 Volume.17 No. 1 p.39 ~ p.44
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Isolation and characterization of Chloroplast DNA in Korean Ginseng Panax ginseng C. A Meyer
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Abstract
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In Korean ginseng. Panax ginseng C. A, Meyer, it was difficult to isolate chloroplast DNA with classical methods, Because of the high polysaccharide content of ginseng chloroplast. The simple and efficient method of chloroplast DNA isolation from
ginseng leaves has been developed by modification recently advanced methods. Also. It can be successfully applied to ctDNA isolation of Chinese cabbage, radish, petunia, tobacco as well as ginseng. Isolated chloroplast DNA from ginseng was
digested
with
various restriction endonucleases. It was estimated that the molecular weight of Korean ginseng chloroplast DNA was about 142kb. There was no difference in difference in restriction endonuclease digestion patterns between two variants of Korean
ginseng
which are Jakyung-Jong (violet-stem variant)and Hwang-sook-Jong (yellow-berry variant)
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KEYWORD
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